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Enzo Biochem bioarray t7 rna polymerase labeling kit
Bioarray T7 Rna Polymerase Labeling Kit, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bioarray+t7+rna+polymerase+labeling+kit/bioarray+t7+rna+polymerase+labeling+kit/pmc05288201-173-16-21
Average 90 stars, based on 1 article reviews
bioarray t7 rna polymerase labeling kit - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Isolation:

Article Title: Nrf2 Regulates an Adaptive Response Protecting Against Oxidative Damage Following Diquat-Mediated Formation of Superoxide Anion
Article Snippet: Briefly, double-stranded cDNA was synthesized from total RNA with SuperScript Choice System (Invitrogen) by using oligo(dT) 24 primers with a T7 RNA polymerase promoter site added to its 3′ end (Genset Corp., La Jolla, CA). .. The isolated cDNA was then labeled to generate biotinylated cRNA in vitro and amplified using the BioArray T7 RNA polymerase labeling kit (Enzo, Farmingdale, NY). .. After purification of the cRNA by RNeasy Mini kit, cRNA were fragmented at 94°C for 35 min. cRNA was used in a hybridization mixture containing herring sperm DNA (0.1 mg/ml; Promega Corp., Madison, WI), plus bacterial and phage cRNA controls (1.5 pmol of BioB, 5 pmol of BioC, 25 pmol of BioD, and 100 pmol of Cre) to serve as internal controls for hybridization efficiency as directed by the manufacturer (Affymetrix).

Article Title: Identification of Transcriptional Targets of HOXA5
Article Snippet: Briefly, double-stranded cDNA was synthesized from 15 g of total RNA with SuperScript Choice System (Invitrogen) by using oligo(dT)24 primers, with a T7 RNA polymerase promoter site added to its 3 -end (Genset Corp, La Jolla, CA). .. The isolated cDNA was then labeled to generate biotinylated cRNA in vitro and amplified using the BioArray T7 RNA polymerase labeling kit (Enzo, Farmingdale, NY). ..

Labeling:

Article Title: Nrf2 Regulates an Adaptive Response Protecting Against Oxidative Damage Following Diquat-Mediated Formation of Superoxide Anion
Article Snippet: Briefly, double-stranded cDNA was synthesized from total RNA with SuperScript Choice System (Invitrogen) by using oligo(dT) 24 primers with a T7 RNA polymerase promoter site added to its 3′ end (Genset Corp., La Jolla, CA). .. The isolated cDNA was then labeled to generate biotinylated cRNA in vitro and amplified using the BioArray T7 RNA polymerase labeling kit (Enzo, Farmingdale, NY). .. After purification of the cRNA by RNeasy Mini kit, cRNA were fragmented at 94°C for 35 min. cRNA was used in a hybridization mixture containing herring sperm DNA (0.1 mg/ml; Promega Corp., Madison, WI), plus bacterial and phage cRNA controls (1.5 pmol of BioB, 5 pmol of BioC, 25 pmol of BioD, and 100 pmol of Cre) to serve as internal controls for hybridization efficiency as directed by the manufacturer (Affymetrix).

Article Title: Identification of Transcriptional Targets of HOXA5
Article Snippet: Briefly, double-stranded cDNA was synthesized from 15 g of total RNA with SuperScript Choice System (Invitrogen) by using oligo(dT)24 primers, with a T7 RNA polymerase promoter site added to its 3 -end (Genset Corp, La Jolla, CA). .. The isolated cDNA was then labeled to generate biotinylated cRNA in vitro and amplified using the BioArray T7 RNA polymerase labeling kit (Enzo, Farmingdale, NY). ..

Article Title: Chemoprevention of oxidative stress-associated oral carcinogenesis by sulforaphane depends on NRF2 and the isothiocyanate moiety
Article Snippet: Briefly, double-stranded cDNA containing T7 promoter (Genset, La Jolla, CA) was synthesized from total RNA using the SuperScript Choice System (Invitrogen). .. Biotinylated cRNAs were generated from cDNAs by in vitro transcription and amplified by using the BioArray T7 RNA polymerase labeling kit (Enzo Diagnostics). .. After purification of cRNAs by GeneChip Sample Cleanup Module (Affymetrix), 15 μg of cRNA was fragmented at 94°C for 35 min.

Article Title: Transgenic mouse model of B cell malignancy
Article Snippet: Briefly, double-stranded cDNA was synthesized from total RNA using the SuperScript Choice System (Invitrogen), which adds a T7 RNA polymerase promoter site to the 3′-end (Genset, La Jolla, Calif.). .. Biotinylated cRNAs were generated from cDNAs in vitro and amplified using the BioArray T7 RNA polymerase labeling kit (Enzo Diagnostics). .. After purification of cRNAs using the RNeasy mini kit (Qiagen, Hilden, Germany), 20 μg of cRNA was fragmented at 94° C. for 35 min.

Article Title: BRCA1 5083del19 Mutant Allele Selectively Up-Regulates Periostin Expression In vitro and In vivo
Article Snippet: Briefly, double-stranded cDNA was synthesized from total RNA with the SuperScript Choice System (Invitrogen), with a T7 RNA polymerase promoter site added to its 3¶-end (Genset). .. Biotinylated cRNAs were generated from cDNAs in vitro and amplified by using the BioArray T7 RNA polymerase labeling kit (Enzo Diagnostics). .. After purification of cRNAs by the RNeasy mini kit (Qiagen), 20 Ag cRNA was fragmented at 94jC for 35 min. Fragmented cRNA (f12.5 Ag) was used in a 250 AL hybridization mixture containing herring-sperm DNA (0.1 mg/mL; Promega) plus bacterial and phage cRNA controls (1.5 pmol/L BioB, 5 pmol/L BioC, 25 pmol/L BioD, and 100 pmol/L Cre) to serve as internal controls for hybridization efficiency.

In Vitro:

Article Title: Nrf2 Regulates an Adaptive Response Protecting Against Oxidative Damage Following Diquat-Mediated Formation of Superoxide Anion
Article Snippet: Briefly, double-stranded cDNA was synthesized from total RNA with SuperScript Choice System (Invitrogen) by using oligo(dT) 24 primers with a T7 RNA polymerase promoter site added to its 3′ end (Genset Corp., La Jolla, CA). .. The isolated cDNA was then labeled to generate biotinylated cRNA in vitro and amplified using the BioArray T7 RNA polymerase labeling kit (Enzo, Farmingdale, NY). .. After purification of the cRNA by RNeasy Mini kit, cRNA were fragmented at 94°C for 35 min. cRNA was used in a hybridization mixture containing herring sperm DNA (0.1 mg/ml; Promega Corp., Madison, WI), plus bacterial and phage cRNA controls (1.5 pmol of BioB, 5 pmol of BioC, 25 pmol of BioD, and 100 pmol of Cre) to serve as internal controls for hybridization efficiency as directed by the manufacturer (Affymetrix).

Article Title: Identification of Transcriptional Targets of HOXA5
Article Snippet: Briefly, double-stranded cDNA was synthesized from 15 g of total RNA with SuperScript Choice System (Invitrogen) by using oligo(dT)24 primers, with a T7 RNA polymerase promoter site added to its 3 -end (Genset Corp, La Jolla, CA). .. The isolated cDNA was then labeled to generate biotinylated cRNA in vitro and amplified using the BioArray T7 RNA polymerase labeling kit (Enzo, Farmingdale, NY). ..

Article Title: Chemoprevention of oxidative stress-associated oral carcinogenesis by sulforaphane depends on NRF2 and the isothiocyanate moiety
Article Snippet: Briefly, double-stranded cDNA containing T7 promoter (Genset, La Jolla, CA) was synthesized from total RNA using the SuperScript Choice System (Invitrogen). .. Biotinylated cRNAs were generated from cDNAs by in vitro transcription and amplified by using the BioArray T7 RNA polymerase labeling kit (Enzo Diagnostics). .. After purification of cRNAs by GeneChip Sample Cleanup Module (Affymetrix), 15 μg of cRNA was fragmented at 94°C for 35 min.

Article Title: Transgenic mouse model of B cell malignancy
Article Snippet: Briefly, double-stranded cDNA was synthesized from total RNA using the SuperScript Choice System (Invitrogen), which adds a T7 RNA polymerase promoter site to the 3′-end (Genset, La Jolla, Calif.). .. Biotinylated cRNAs were generated from cDNAs in vitro and amplified using the BioArray T7 RNA polymerase labeling kit (Enzo Diagnostics). .. After purification of cRNAs using the RNeasy mini kit (Qiagen, Hilden, Germany), 20 μg of cRNA was fragmented at 94° C. for 35 min.

Article Title: BRCA1 5083del19 Mutant Allele Selectively Up-Regulates Periostin Expression In vitro and In vivo
Article Snippet: Briefly, double-stranded cDNA was synthesized from total RNA with the SuperScript Choice System (Invitrogen), with a T7 RNA polymerase promoter site added to its 3¶-end (Genset). .. Biotinylated cRNAs were generated from cDNAs in vitro and amplified by using the BioArray T7 RNA polymerase labeling kit (Enzo Diagnostics). .. After purification of cRNAs by the RNeasy mini kit (Qiagen), 20 Ag cRNA was fragmented at 94jC for 35 min. Fragmented cRNA (f12.5 Ag) was used in a 250 AL hybridization mixture containing herring-sperm DNA (0.1 mg/mL; Promega) plus bacterial and phage cRNA controls (1.5 pmol/L BioB, 5 pmol/L BioC, 25 pmol/L BioD, and 100 pmol/L Cre) to serve as internal controls for hybridization efficiency.

Amplification:

Article Title: Nrf2 Regulates an Adaptive Response Protecting Against Oxidative Damage Following Diquat-Mediated Formation of Superoxide Anion
Article Snippet: Briefly, double-stranded cDNA was synthesized from total RNA with SuperScript Choice System (Invitrogen) by using oligo(dT) 24 primers with a T7 RNA polymerase promoter site added to its 3′ end (Genset Corp., La Jolla, CA). .. The isolated cDNA was then labeled to generate biotinylated cRNA in vitro and amplified using the BioArray T7 RNA polymerase labeling kit (Enzo, Farmingdale, NY). .. After purification of the cRNA by RNeasy Mini kit, cRNA were fragmented at 94°C for 35 min. cRNA was used in a hybridization mixture containing herring sperm DNA (0.1 mg/ml; Promega Corp., Madison, WI), plus bacterial and phage cRNA controls (1.5 pmol of BioB, 5 pmol of BioC, 25 pmol of BioD, and 100 pmol of Cre) to serve as internal controls for hybridization efficiency as directed by the manufacturer (Affymetrix).

Article Title: Identification of Transcriptional Targets of HOXA5
Article Snippet: Briefly, double-stranded cDNA was synthesized from 15 g of total RNA with SuperScript Choice System (Invitrogen) by using oligo(dT)24 primers, with a T7 RNA polymerase promoter site added to its 3 -end (Genset Corp, La Jolla, CA). .. The isolated cDNA was then labeled to generate biotinylated cRNA in vitro and amplified using the BioArray T7 RNA polymerase labeling kit (Enzo, Farmingdale, NY). ..

Article Title: Chemoprevention of oxidative stress-associated oral carcinogenesis by sulforaphane depends on NRF2 and the isothiocyanate moiety
Article Snippet: Briefly, double-stranded cDNA containing T7 promoter (Genset, La Jolla, CA) was synthesized from total RNA using the SuperScript Choice System (Invitrogen). .. Biotinylated cRNAs were generated from cDNAs by in vitro transcription and amplified by using the BioArray T7 RNA polymerase labeling kit (Enzo Diagnostics). .. After purification of cRNAs by GeneChip Sample Cleanup Module (Affymetrix), 15 μg of cRNA was fragmented at 94°C for 35 min.

Article Title: Transgenic mouse model of B cell malignancy
Article Snippet: Briefly, double-stranded cDNA was synthesized from total RNA using the SuperScript Choice System (Invitrogen), which adds a T7 RNA polymerase promoter site to the 3′-end (Genset, La Jolla, Calif.). .. Biotinylated cRNAs were generated from cDNAs in vitro and amplified using the BioArray T7 RNA polymerase labeling kit (Enzo Diagnostics). .. After purification of cRNAs using the RNeasy mini kit (Qiagen, Hilden, Germany), 20 μg of cRNA was fragmented at 94° C. for 35 min.

Article Title: BRCA1 5083del19 Mutant Allele Selectively Up-Regulates Periostin Expression In vitro and In vivo
Article Snippet: Briefly, double-stranded cDNA was synthesized from total RNA with the SuperScript Choice System (Invitrogen), with a T7 RNA polymerase promoter site added to its 3¶-end (Genset). .. Biotinylated cRNAs were generated from cDNAs in vitro and amplified by using the BioArray T7 RNA polymerase labeling kit (Enzo Diagnostics). .. After purification of cRNAs by the RNeasy mini kit (Qiagen), 20 Ag cRNA was fragmented at 94jC for 35 min. Fragmented cRNA (f12.5 Ag) was used in a 250 AL hybridization mixture containing herring-sperm DNA (0.1 mg/mL; Promega) plus bacterial and phage cRNA controls (1.5 pmol/L BioB, 5 pmol/L BioC, 25 pmol/L BioD, and 100 pmol/L Cre) to serve as internal controls for hybridization efficiency.

Generated:

Article Title: Chemoprevention of oxidative stress-associated oral carcinogenesis by sulforaphane depends on NRF2 and the isothiocyanate moiety
Article Snippet: Briefly, double-stranded cDNA containing T7 promoter (Genset, La Jolla, CA) was synthesized from total RNA using the SuperScript Choice System (Invitrogen). .. Biotinylated cRNAs were generated from cDNAs by in vitro transcription and amplified by using the BioArray T7 RNA polymerase labeling kit (Enzo Diagnostics). .. After purification of cRNAs by GeneChip Sample Cleanup Module (Affymetrix), 15 μg of cRNA was fragmented at 94°C for 35 min.

Article Title: Transgenic mouse model of B cell malignancy
Article Snippet: Briefly, double-stranded cDNA was synthesized from total RNA using the SuperScript Choice System (Invitrogen), which adds a T7 RNA polymerase promoter site to the 3′-end (Genset, La Jolla, Calif.). .. Biotinylated cRNAs were generated from cDNAs in vitro and amplified using the BioArray T7 RNA polymerase labeling kit (Enzo Diagnostics). .. After purification of cRNAs using the RNeasy mini kit (Qiagen, Hilden, Germany), 20 μg of cRNA was fragmented at 94° C. for 35 min.

Article Title: BRCA1 5083del19 Mutant Allele Selectively Up-Regulates Periostin Expression In vitro and In vivo
Article Snippet: Briefly, double-stranded cDNA was synthesized from total RNA with the SuperScript Choice System (Invitrogen), with a T7 RNA polymerase promoter site added to its 3¶-end (Genset). .. Biotinylated cRNAs were generated from cDNAs in vitro and amplified by using the BioArray T7 RNA polymerase labeling kit (Enzo Diagnostics). .. After purification of cRNAs by the RNeasy mini kit (Qiagen), 20 Ag cRNA was fragmented at 94jC for 35 min. Fragmented cRNA (f12.5 Ag) was used in a 250 AL hybridization mixture containing herring-sperm DNA (0.1 mg/mL; Promega) plus bacterial and phage cRNA controls (1.5 pmol/L BioB, 5 pmol/L BioC, 25 pmol/L BioD, and 100 pmol/L Cre) to serve as internal controls for hybridization efficiency.



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